Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing.

ABSTRACT:

Background

Understanding the dynamics of gametocyte production in polyclonal Plasmodium falciparum infections requires a genotyping method that detects distinct gametocyte clones and estimates their relative frequencies. Here, a marker was identified and evaluated to genotype P. falciparum mature gametocytes using amplicon deep sequencing.

Methods

A data set of polymorphic regions of the P. falciparum genome was mined to identify a gametocyte genotyping marker. To assess marker resolution, the number of unique haplotypes in the marker region was estimated from 95 Malawian P. falciparum whole genome sequences. Specificity of the marker for detection of mature gametocytes was evaluated using reverse transcription-polymerase chain reaction of RNA extracted from NF54 mature game

SUBMITTER: Vareta J 

PROVIDER: S-EPMC10999092 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature