Calcium image analysis in the moving gut.

ABSTRACT:

Background

The neural control of gastrointestinal muscle relies on circuit activity whose underlying motifs remain limited by small-sample calcium imaging recordings confounded by motion artifact, paralytics, and muscle dissections. We present a sequence of resources to register images from moving preparations and identify out-of-focus events in widefield fluorescent microscopy.

Methods

Our algorithm uses piecewise rigid registration with pathfinding to correct movements associated with smooth muscle contractions. We developed methods to identify loss-of-focus events and to simulate calcium activity to evaluate registration.

Key results

By combining our methods with principal component analysis, we found populations of neurons exhibit distinct activity patterns in res

SUBMITTER: Barth BB 

PROVIDER: S-EPMC10999186 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature