CHRFAM7A diversifies human immune adaption through Ca²⁺ signalling and actin cytoskeleton reorganization.

ABSTRACT:

Background

Human restricted genes contribute to human specific traits in the immune system. CHRFAM7A, a uniquely human fusion gene, is a negative regulator of the α7 nicotinic acetylcholine receptor (α7 nAChR), the highest Ca²⁺ conductor of the ACh receptors implicated in innate immunity. Understanding the mechanism of how CHRFAM7A affects the immune system remains unexplored.

Methods

Two model systems are used, human induced pluripotent stem cells (iPSC) and human primary monocytes, to characterize α7 nAChR function, Ca²⁺ dynamics and decoders to elucidate the pathway from receptor to phenotype.

Findings

CHRFAM7A/α7 nAChR is identified as a hypomorphic receptor with mitigated Ca²⁺ influx and prolonged channel closed state. This shifts

SUBMITTER: Szigeti K 

PROVIDER: S-EPMC10999709 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature