Substrate specificity of a ketosynthase domain involved in bacillaene biosynthesis.

ABSTRACT: An isotopic labelling method was developed to investigate substrate binding by ketosynthases, exemplified by the second ketosynthase of the polyketide synthase BaeJ involved in bacillaene biosynthesis (BaeJ-KS2). For this purpose, both enantiomers of a ¹³C-labelled N-acetylcysteamine thioester (SNAC ester) surrogate of the proposed natural intermediate of BaeJ-KS2 were synthesised, including an enzymatic step with glutamate decarboxylase, and incubated with BaeJ-KS2. Substrate binding was demonstrated through ¹³C NMR analysis of the products against the background of various control experiments.

SUBMITTER: Yin Z 

PROVIDER: S-EPMC10999986 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature