Blind instrument response function identification from fluorescence decays.

ABSTRACT: Time-resolved fluorescence spectroscopy plays a crucial role when studying dynamic properties of complex photochemical systems. Nevertheless, the analysis of measured time decays and the extraction of exponential lifetimes often requires either the experimental assessment or the modeling of the instrument response function (IRF). However, the intrinsic nature of the IRF in the measurement process, which may vary across measurements due to chemical and instrumental factors, jeopardizes the results obtained by reconvolution approaches. In this paper, we introduce a novel methodology, called blind instrument response function identification (BIRFI), which enables the direct estimation of the IRF from the collected data. It capitalizes on the properties of single exponential signals to transfo

SUBMITTER: Gomez-Sanchez A 

PROVIDER: S-EPMC11000113 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature