CDC45 promotes the stemness and metastasis in lung adenocarcinoma by affecting the cell cycle.

ABSTRACT:

Objective

This study aimed to assess the functions of cell division cycle protein 45 (CDC45) in Non-small cell lung cancer (NSCLC) cancer and its effects on stemness and metastasis.

Methods

Firstly, differentially expressed genes related to lung cancer metastasis and stemness were screened by differential analysis and lasso regression. Then, in vitro, experiments such as colony formation assay, scratch assay, and transwell assay were conducted to evaluate the impact of CDC45 knockdown on the proliferation and migration abilities of lung cancer cells. Western blotting was used to measure the expression levels of related proteins and investigate the regulation of CDC45 on the cell cycle. Finally, in vivo model with subcutaneous injection of lung cancer cells was performed to v

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11000299 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature