Enriched Single-Nucleus RNA-Sequencing Reveals Unique Attributes of Distal Convoluted Tubule Cells.

ABSTRACT:

Significance statement

High-resolution single-nucleus RNA-sequencing data indicate a clear separation between primary sites of calcium and magnesium handling within distal convoluted tubule (DCT). Both DCT1 and DCT2 express Slc12a3, but these subsegments serve distinctive functions, with more abundant magnesium-handling genes along DCT1 and more calcium-handling genes along DCT2. The data also provide insight into the plasticity of the distal nephron-collecting duct junction, formed from cells of separate embryonic origins. By focusing/changing gradients of gene expression, the DCT can morph into different physiological cell states on demand.

Background

The distal convoluted tubule (DCT) comprises two subsegments, DCT1 and DCT2, with different functional and molecular charac

SUBMITTER: Su XT 

PROVIDER: S-EPMC11000721 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature