Restricted glycolysis is a primary cause of the reduced growth rate of zinc-deficient yeast cells.

ABSTRACT: Zinc is required for many critical processes, including intermediary metabolism. In Saccharomyces cerevisiae, the Zap1 activator regulates the transcription of ∼80 genes in response to Zn supply. Some Zap1-regulated genes are Zn transporters that maintain Zn homeostasis, while others mediate adaptive responses that enhance fitness. One adaptive response gene encodes the 2-cysteine peroxiredoxin Tsa1, which is critical to Zn-deficient (ZnD) growth. Depending on its redox state, Tsa1 can function as a peroxidase, a protein chaperone, or a regulatory redox sensor. In a screen for possible Tsa1 regulatory targets, we identified a mutation (cdc19^S492A) that partially suppressed the tsa1Δ growth defect. The cdc19^S492A mutation reduced activity of its protein product, pyruva

SUBMITTER: MacDiarmid CW 

PROVIDER: S-EPMC11001634 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature