Enhancing Escherichia coli abiotic stress resistance through ornithine lipid formation.

ABSTRACT: Escherichia coli is a common host for biotechnology and synthetic biology applications. During growth and fermentation, the microbes are often exposed to stress conditions, such as variations in pH or solvent concentrations. Bacterial membranes play a key role in response to abiotic stresses. Ornithine lipids (OLs) are a group of membrane lipids whose presence and synthesis have been related to stress resistance in bacteria. We wondered if this stress resistance could be transferred to bacteria not encoding the capacity to form OLs in their genome, such as E. coli. In this study, we engineered different E. coli strains to produce unmodified OLs and hydroxylated OLs by expressing the synthetic operon olsFC. Our results showed that OL formation improved pH resistance and increased biomass un

SUBMITTER: Bedoya-Perez LP 

PROVIDER: S-EPMC11001654 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature