Differential proteomics profile of microcapillary networks in response to sound pattern-driven local cell density enhancement.

ABSTRACT: Spatial cell organization and biofabrication of microcapillary networks in vitro has a great potential in tissue engineering and regenerative medicine. This study explores the impact of local cell density enhancement achieved through an innovative sound-based patterning on microcapillary networks formation and their proteomic profile. Human umbilical vein endothelial cells (HUVEC) and human pericytes from placenta (hPC-PL) were mixed in a fibrin suspension. The mild effect of sound-induced hydrodynamic forces condensed cells into architected geometries showing good fidelity to the numerical simulation of the physical process. Local cell density increased significantly within the patterned areas and the capillary-like structures formed following the cell density gradient. Over five days, th

SUBMITTER: Di Marzio N 

PROVIDER: S-EPMC11001772 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature