Evaluation of specific RBE in different cells of hippocampus under high-dose proton irradiation in rats.

ABSTRACT: The study aimed to determine the specific relative biological effectiveness (RBE) of various cells in the hippocampus following proton irradiation. Sixty Sprague-Dawley rats were randomly allocated to 5 groups receiving 20 or 30 Gy of proton or photon irradiation. Pathomorphological neuronal damage in the hippocampus was assessed using Hematoxylin-eosin (HE) staining. The expression level of NeuN, Nestin, Caspase-3, Olig2, CD68 and CD45 were determined by immunohistochemistry (IHC). The RBE range established by comparing the effects of proton and photon irradiation at equivalent biological outcomes. Proton_20Gy induced more severe damage to neurons than photon_20Gy, but showed no difference compared to photon_30Gy. The RBE of neuron was determined to be 1.65.

SUBMITTER: Zhou S 

PROVIDER: S-EPMC11001863 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature