A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell.

ABSTRACT: Cell surface proteins are responsible for many crucial physiological roles, and they are also the major category of drug targets as the majority of therapeutics target membrane proteins on the surface of cells to alter cellular signaling. Despite its great significance, ligand discovery against membrane proteins has posed a great challenge mainly due to the special property of their natural habitat. Here, we design a new chemical proteomic probe OPA-S-S-alkyne that can efficiently and selectively target the lysines exposed on the cell surface and develop a chemical proteomics strategy for global analysis of surface functionality (GASF) in living cells. In total, we quantified 2639 cell surface lysines in Hela cell and several hundred residues with high reactivity were discovered, which rep

SUBMITTER: Wang T 

PROVIDER: S-EPMC11001985 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature