The role of influenza A virus hemagglutinin residues 226 and 228 in receptor specificity and host range restriction.

ABSTRACT: Influenza A viruses can be isolated from a variety of animals, but their range of hosts is restricted. For example, human influenza viruses do not replicate in duck intestine, the major replication site of avian viruses in ducks. Although amino acids at positions 226 and 228 of hemagglutinin (HA) of the H3 subtype are known to be important for this host range restriction, the contributions of specific amino acids at these positions to restriction were not known. Here, we address this issue by generating HAs with site-specific mutations of a human virus that contain different amino acid residues at these positions. We also let ducks select replication-competent viruses from a replication-incompetent virus containing a human virus HA by inoculating animals with 10(10.5) 50% egg infectious do

SUBMITTER: Vines A 

PROVIDER: S-EPMC110023 | biostudies-literature | 1998 Sep

REPOSITORIES: biostudies-literature