Plumbagin induces ferroptosis in colon cancer cells by regulating p53-related SLC7A11 expression.

ABSTRACT:

Objective

This study examined the mechanism through which plumbagin induces ferroptosis of colon cancer cells.

Methods

CCK-8 assay was performed to examine the viability of colon cancer cells (SW480 and HCT116 cells) after they were treated with 0-, 5-, 10-, 15- and 20-μmol/L plumbagin. Colony formation assay and Transwell assay were used to examine the effects of 15-μmol/L plumbagin on the proliferation, invasive ability. The ferroptosis of SW480 and HCT116 cells and the expression of p-p53, p53 and SLC7A11 were analysed. The effects of blocking necrosis, apoptosis and ferroptosis on the anti-cancer effects of plumbagin were examined. After p53 was silenced, the effects of plumbagin on proliferation, invasion, ferroptosis and SLC7A11 expression were assessed. A tumour-beari

SUBMITTER: Wang B 

PROVIDER: S-EPMC11002553 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature