CD14 facilitates perinatal human cytomegalovirus infection in biliary epithelial cells via CD55.

ABSTRACT:

Background & aims

A high human cytomegalovirus (HCMV) infection rate accompanied by an increased level of bile duct damage is observed in the perinatal period. The possible mechanism was investigated.

Methods

A total of 1,120 HCMV-positive and 9,297 HCMV-negative children were recruited, and depending on age, their liver biochemistry profile was compared. Fetal and infant biliary epithelial cells (F-BECs and I-BECs, respectively) were infected with HCMV, and the differences in cells were revealed by proteomic analysis. Protein-protein interactions were examined by coimmunoprecipitation and mass spectrometry analyses. A murine cytomegalovirus (MCMV) infection model was established to assess treatment effects.

Results

Perinatal HCMV infection significantly increased the

SUBMITTER: Su L 

PROVIDER: S-EPMC11002872 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature