A targeted proteomics method for quantifying plasma apolipoprotein kinetics in individual mice using stable isotope labeling.

ABSTRACT: Altered apolipoprotein kinetics play a critical role in promoting dyslipidemia and atherogenesis. Human apolipoprotein kinetics have been extensively evaluated, but similar studies in mice are hampered by the lack of robust methods suitable for the small amounts of blood that can be collected at sequential time points from individual mice. We describe a targeted liquid chromatography tandem mass spectrometry method for simultaneously quantifying the stable isotope enrichment of several apolipoproteins represented by multiple peptides in serial blood samples (15 μl each) obtained after retro-orbital injection of ¹³C₆,¹⁵N₂-lysine (Lys8) in mice. We determined apolipoprotein fractional clearance rates (FCRs) and production rates (PRs) in WT mice and

SUBMITTER: Shao B 

PROVIDER: S-EPMC11002879 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature