Histone lactylation in macrophages is predictive for gene expression changes during ischemia induced-muscle regeneration.

ABSTRACT:

Objectives

We have previously shown that lactate is an essential metabolite for macrophage polarisation during ischemia-induced muscle regeneration. Recent in vitro work has implicated histone lactylation, a direct derivative of lactate, in macrophage polarisation. Here, we explore the in vivo relevance of histone lactylation for macrophage polarisation after muscle injury.

Methods

To evaluate macrophage dynamics during muscle regeneration, we subjected mice to ischemia-induced muscle damage by ligating the femoral artery. Muscle samples were harvested at 1, 2, 4, and 7 days post injury (dpi). CD45⁺CD11b⁺F4/80⁺CD64⁺ macrophages were isolated and processed for RNA sequencing, Western Blotting, and CUT&Tag-sequencing to investigate g

SUBMITTER: Desgeorges T 

PROVIDER: S-EPMC11002880 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature