Fluorescent tools for the standardized work in Gram-negative bacteria.

ABSTRACT: Standardized and thoroughly characterized genetic tools are a prerequisite for studying cellular processes to ensure the reusability and consistency of experimental results. The discovery of fluorescent proteins (FPs) represents a milestone in the development of genetic reporters for monitoring transcription or protein localization in vivo. FPs have revolutionized our understanding of cellular dynamics by enabling the real-time visualization and tracking of biological processes. Despite these advancements, challenges remain in the appropriate use of FPs, specifically regarding their proper application, protein turnover dynamics, and the undesired disruption of cellular functions. Here, we systematically compared a comprehensive set of 15 FPs and assessed their performance in vivo by focusi

SUBMITTER: Delgadillo-Guevara M 

PROVIDER: S-EPMC11003136 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature