A phenotypic screening approach to target p60AmotL2-expressing invasive cancer cells.

ABSTRACT:

Background

Tumor cells have the ability to invade and form small clusters that protrude into adjacent tissues, a phenomenon that is frequently observed at the periphery of a tumor as it expands into healthy tissues. The presence of these clusters is linked to poor prognosis and has proven challenging to treat using conventional therapies. We previously reported that p60AmotL2 expression is localized to invasive colon and breast cancer cells. In vitro, p60AmotL2 promotes epithelial cell invasion by negatively impacting E-cadherin/AmotL2-related mechanotransduction.

Methods

Using epithelial cells transfected with inducible p60AmotL2, we employed a phenotypic drug screening approach to find compounds that specifically target invasive cells. The phenotypic screen was performed b

SUBMITTER: Fonseca P 

PROVIDER: S-EPMC11003180 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature