CRISPR screens decode cancer cell pathways that trigger γδ T cell detection.

ABSTRACT: γδ T cells are potent anticancer effectors with the potential to target tumours broadly, independent of patient-specific neoantigens or human leukocyte antigen background^1-5. γδ T cells can sense conserved cell stress signals prevalent in transformed cells^2,3, although the mechanisms behind the targeting of stressed target cells remain poorly characterized. Vγ9Vδ2 T cells-the most abundant subset of human γδ T cells⁴-recognize a protein complex containing butyrophilin 2A1 (BTN2A1) and BTN3A1 (refs. ^6-8), a widely expressed cell surface protein that is activated by phosphoantigens abundantly produced by tumour cells. Here we combined genome-wide CRISPR screens in target cancer cells to identify pathways that regulate γδ T cell killing and BTN3A ce

SUBMITTER: Mamedov MR 

PROVIDER: S-EPMC11003766 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature