Temperature-modulated separation of vascular cells using thermoresponsive-anionic block copolymer-modified glass.

ABSTRACT:

Introduction

Vascular tissue engineering is a key technology in the field of regenerative medicine. In tissue engineering, the separation of vascular cells without cell modification is required, as cell modifications affect the intrinsic properties of the cells. In this study, we have developed an effective method for separating vascular cells without cell modification, using a thermoresponsive anionic block copolymer.

Methods

A thermoresponsive anionic block copolymer, poly(acrylic acid)-b-poly(N-isopropylacryl-amide) (PAAc-b-PNIPAAm), with various PNIPAAm segment lengths, was prepared in two steps: atom transfer radical polymerization and subsequent deprotection. Normal human umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts, a

SUBMITTER: Hirotani T 

PROVIDER: S-EPMC11004074 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature