An MR-based brain template and atlas for optical projection tomography and light sheet fluorescence microscopy in neuroscience.

ABSTRACT:

Introduction

Optical Projection Tomography (OPT) and light sheet fluorescence microscopy (LSFM) are high resolution optical imaging techniques, ideally suited for ex vivo 3D whole mouse brain imaging. Although they exhibit high specificity for their targets, the anatomical detail provided by tissue autofluorescence remains limited.

Methods

T1-weighted images were acquired from 19 BABB or DBE cleared brains to create an MR template using serial longitudinal registration. Afterwards, fluorescent OPT and LSFM images were coregistered/normalized to the MR template to create fusion images.

Results

Volumetric calculations revealed a significant difference between BABB and DBE cleared brains, leading to develop two optimized templates, with associated tissue priors and brain

SUBMITTER: Willekens SMA 

PROVIDER: S-EPMC11004350 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature