Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy.

ABSTRACT: Centriolar satellites are high-order assemblies, scaffolded by the protein PCM1, that gravitate as particles around the centrosome and play pivotal roles in fundamental cellular processes notably ciliogenesis and autophagy. Despite stringent control mechanisms involving phosphorylation and ubiquitination, the landscape of post-translational modifications shaping these structures remains elusive. Here, we report that necrosulfonamide (NSA), a small molecule known for binding and inactivating the pivotal effector of cell death by necroptosis MLKL, intersects with centriolar satellites, ciliogenesis, and autophagy independently of MLKL. NSA functions as a potent redox cycler and triggers the oxidation and aggregation of PCM1 alongside select partners, while minimally impacting the overall dis

SUBMITTER: Renaud CCN 

PROVIDER: S-EPMC11004361 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature