Methionyl-tRNA synthetase synthetic and proofreading activities are determinants of antibiotic persistence.

ABSTRACT: Bacterial antibiotic persistence is a phenomenon where bacteria are exposed to an antibiotic and the majority of the population dies while a small subset enters a low metabolic, persistent, state and are able to survive. Once the antibiotic is removed the persistent population can resuscitate and continue growing. Several different molecular mechanisms and pathways have been implicated in this phenomenon. A common mechanism that may underly bacterial antibiotic persistence is perturbations in protein synthesis. To investigate this mechanism, we characterized four distinct metG mutants for their ability to increase antibiotic persistence. Two metG mutants encode changes near the catalytic site of MetRS and the other two mutants changes near the anticodon binding domain. Mutati

SUBMITTER: Wood WN 

PROVIDER: S-EPMC11004401 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature