Identifying the suite of genes central to swimming in the biocontrol bacterium Pseudomonas protegens Pf-5.

ABSTRACT: Swimming motility is a key bacterial trait, important to success in many niches. Biocontrol bacteria, such as Pseudomonas protegens Pf-5, are increasingly used in agriculture to control crop diseases, where motility is important for colonization of the plant rhizosphere. Swimming motility typically involves a suite of flagella and chemotaxis genes, but the specific gene set employed for both regulation and biogenesis can differ substantially between organisms. Here we used transposon-directed insertion site sequencing (TraDIS), a genome-wide approach, to identify 249 genes involved in P. protegens Pf-5 swimming motility. In addition to the expected flagella and chemotaxis, we also identified a suite of additional genes important for swimming, including genes related to peptid

SUBMITTER: Fabian BK 

PROVIDER: S-EPMC11004494 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature