Elucidating the role of EPPK1 in lung adenocarcinoma development.

ABSTRACT:

Background

We recently found that epiplakin 1 (EPPK1) alterations were present in 12% of lung adenocarcinoma (LUAD) cases and were associated with a poor prognosis in early-stage LUAD when combined with other molecular alterations. This study aimed to identify a probable crucial role for EPPK1 in cancer development.

Methods

EPPK1 mRNA and protein expression was analyzed with clinical variables. Normal bronchial epithelial cell lines were exposed to cigarette smoke for 16 weeks to determine whether EPPK1 protein expression was altered after exposure. Further, we used CRISPR-Cas9 to knock out (KO) EPPK1 in LUAD cell lines and observed how the cancer cells were altered functionally and genetically.

Results

EPPK1 protein expression was associated with smoking and poor pro

SUBMITTER: Arimura K 

PROVIDER: S-EPMC11005125 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature