Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc² 155 expressing mammalian steroidogenesis system.

ABSTRACT:

Background

Pregnenolone and progesterone are the life-important steroid hormones regulating essential vital functions in mammals, and widely used in different fields of medicine. Microbiological production of these compounds from sterols is based on the use of recombinant strains expressing the enzyme system cholesterol hydroxylase/C20-C22 lyase (CH/L) of mammalian steroidogenesis. However, the efficiency of the known recombinant strains is still low. New recombinant strains and combination approaches are now needed to produce these steroid hormones.

Results

Based on Mycolicibacterium smegmatis, a recombinant strain was created that expresses the steroidogenesis system (CYP11A1, adrenodoxin reductase, adrenodoxin) of the bovine adrenal cortex. The recombinant strain transfor

SUBMITTER: Karpov M 

PROVIDER: S-EPMC11005228 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature