Proteins a la carte: riboproteogenomic exploration of bacterial N-terminal proteoform expression.

ABSTRACT: In recent years, it has become evident that the true complexity of bacterial proteomes remains underestimated. Gene annotation tools are known to propagate biases and overlook certain classes of truly expressed proteins, particularly proteoforms-protein isoforms arising from a single gene. Recent (re-)annotation efforts heavily rely on ribosome profiling by providing a direct readout of translation to fully describe bacterial proteomes. In this study, we employ a robust riboproteogenomic pipeline to conduct a systematic census of expressed N-terminal proteoform pairs, representing two isoforms encoded by a single gene raised by annotated and alternative translation initiation, in Salmonella. Intriguingly, conditional-dependent changes in relative utilization of annotated and alterna

SUBMITTER: Fijalkowski I 

PROVIDER: S-EPMC11005335 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature