Strengthening Bordetella pertussis genomic surveillance by direct sequencing of residual positive specimens.

ABSTRACT: Whole-genome sequencing (WGS) of microbial pathogens recovered from patients with infectious disease facilitates high-resolution strain characterization and molecular epidemiology. However, increasing reliance on culture-independent methods to diagnose infectious diseases has resulted in few isolates available for WGS. Here, we report a novel culture-independent approach to genome characterization of Bordetella pertussis, the causative agent of pertussis and a paradigm for insufficient genomic surveillance due to limited culture of clinical isolates. Sequencing libraries constructed directly from residual pertussis-positive diagnostic nasopharyngeal specimens were hybridized with biotinylated RNA "baits" targeting B. pertussis fragments within complex mixtures that contained

SUBMITTER: Peng Y 

PROVIDER: S-EPMC11005353 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature