Deciphering the regulatory landscape of murine splenic response to anemic stress at single-cell resolution.

ABSTRACT:

Abstract

Stress erythropoiesis can be influenced by multiple mediators through both intrinsic and extrinsic mechanisms in early erythroid precursors. Single-cell RNA sequencing was conducted on spleen tissue isolated from mice subjected to phenylhydrazine and serial bleeding to explore novel molecular mechanisms of stress erythropoiesis. Our results showed prominent emergence of early erythroblast populations under both modes of anemic stress. Analysis of gene expression revealed distinct phases during the development of emerging erythroid cells. Interestingly, we observed the presence of a "hiatus" subpopulation characterized by relatively low level of transcriptional activities that transitions between early stages of emerging erythroid cells, with moderate protein synthesis acti

SUBMITTER: Yang C 

PROVIDER: S-EPMC11006810 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature