In vitro aging alters the gene expression and secretome composition of canine adipose-derived mesenchymal stem cells.

ABSTRACT:

Introduction

Canine adipose-derived mesenchymal stem cells (cAD-MSCs) hold therapeutic promise due to their regenerative potential, particularly within their secretome. However, concerns arise regarding the impact of in vitro cultivation necessitated for storing therapeutic doses, prompting this study to comprehensively explore the impact of in vitro aging on gene expression and secretome composition.

Methods

The study involved collecting abdominal adipose tissue samples from nine healthy female dogs, from which cAD-MSCs were extracted and cultured. Stem cells were validated through trilineage differentiation assays and flow cytometry immunophenotyping. Gene expression profiling using RT-qPCR array, and cAD-MSCs secretome LC-MS/MS analysis, were conducted at pa

SUBMITTER: Prislin M 

PROVIDER: S-EPMC11006985 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature