A putative design for the electromagnetic activation of split proteins for molecular and cellular manipulation.

ABSTRACT: The ability to manipulate cellular function using an external stimulus is a powerful strategy for studying complex biological phenomena. One approach to modulate the function of the cellular environment is split proteins. In this method, a biologically active protein or an enzyme is fragmented so that it reassembles only upon a specific stimulus. Although many tools are available to induce these systems, nature has provided other mechanisms to expand the split protein toolbox. Here, we show a novel method for reconstituting split proteins using magnetic stimulation. We found that the electromagnetic perceptive gene (EPG) changes conformation due to magnetic field stimulation. By fusing split fragments of a certain protein to both termini of the EPG, the fragments can be reassembled into a

SUBMITTER: Grady CJ 

PROVIDER: S-EPMC11007078 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature