High throughput AS LNA qPCR method for the detection of a specific mutation in poliovirus vaccine strains.

ABSTRACT: Sabin Inactivated Poliovirus Vaccine (sIPV) has become one of the preferred vaccination options for the last step in the Poliovirus eradication program. Sequencing of poliovirus samples is needed during the manufacturing of poliovirus vaccines to assure the safety and immunogenicity of these vaccines. Next-generation sequencing analysis is the current costly and time-consuming gold standard for monitoring the manufacturing processes. We developed a low-cost and quick, highly sensitive, and allele-specific locked nucleic acid-probe-based reverse transcription quantitative PCR alternative that can accurately detect mutations in poliovirus vaccine samples during process development, scaling up, and release. Using the frequently in vitro occurring and viral replication-impacting VP1-E_295<

SUBMITTER: Opmeer L 

PROVIDER: S-EPMC11007389 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature