HERC5 downregulation in non-small cell lung cancer is associated with altered energy metabolism and metastasis.

ABSTRACT:

Background

Metastasis is the leading cause of cancer-related death in non-small cell lung cancer (NSCLC) patients. We previously showed that low HERC5 expression predicts early tumor dissemination and a dismal prognosis in NSCLC patients. Here, we performed functional studies to unravel the mechanism underlying the "metastasis-suppressor" effect of HERC5, with a focus on mitochondrial metabolism pathways.

Methods

We assessed cell proliferation, colony formation potential, anchorage-independent growth, migration, and wound healing in NSCLC cell line models with HERC5 overexpression (OE) or knockout (KO). To study early tumor cell dissemination, we used these cell line models in zebrafish experiments and performed intracardial injections in nude mice. Mass spectrometry (MS) wa

SUBMITTER: Schneegans S 

PROVIDER: S-EPMC11008035 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature