Development of a customizable mouse backbone spectral flow cytometry panel to delineate immune cell populations in normal and tumor tissues.

ABSTRACT:

Introduction

In vivo studies of cancer biology and assessment of therapeutic efficacy are critical to advancing cancer research and ultimately improving patient outcomes. Murine cancer models have proven to be an invaluable tool in pre-clinical studies. In this context, multi-parameter flow cytometry is a powerful method for elucidating the profile of immune cells within the tumor microenvironment and/or play a role in hematological diseases. However, designing an appropriate multi-parameter panel to comprehensively profile the increasing diversity of immune cells across different murine tissues can be extremely challenging.

Methods

To address this issue, we designed a panel with 13 fixed markers that define the major immune populations -referred to as the backbone pa

SUBMITTER: Longhini ALF 

PROVIDER: S-EPMC11008467 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature