Targeted Quantification of Proteoforms in Complex Samples by Proteoform Reaction Monitoring.

ABSTRACT: Existing mass spectrometric assays used for sensitive and specific measurements of target proteins across multiple samples, such as selected/multiple reaction monitoring (SRM/MRM) or parallel reaction monitoring (PRM), are peptide-based methods for bottom-up proteomics. Here, we describe an approach based on the principle of PRM for the measurement of intact proteoforms by targeted top-down proteomics, termed proteoform reaction monitoring (PfRM). We explore the ability of our method to circumvent traditional limitations of top-down proteomics, such as sensitivity and reproducibility. We also introduce a new software program, Proteoform Finder (part of ProSight Native), specifically designed for the easy analysis of PfRM data. PfRM was initially benchmarked by quantifying three standard pr

SUBMITTER: Huang CF 

PROVIDER: S-EPMC11008684 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature