In silico identification of papaya genome-encoded microRNAs to target begomovirus genes in papaya leaf curl disease.

ABSTRACT: Papaya leaf curl disease (PaLCuD) is widespread and classified in the genus begomovirus (Geminiviridae), disseminated by the vector whitefly Bemisia tabaci. RNA interference (RNAi)-based antiviral innate immunity stands as a pivotal defense mechanism and biological process in limiting viral genomes to manage plant diseases. The current study aims to identify and analyze Carica Papaya locus-derived capa-microRNAs with predicted potential for targeting divergent begomovirus species-encoded mRNAs using a 'four integrative in silico algorithms' approach. This research aims to experimentally activate the RNAi catalytic pathway using in silico-predicted endogenous capa-miRNAs and create papaya varieties capable of assessing potential resistance against b

SUBMITTER: Srivastava A 

PROVIDER: S-EPMC11008722 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature