Endoplasmic Reticulum Stress and Unfolded Protein Response in Vernal Keratoconjunctivitis.

ABSTRACT:

Purpose

Vernal keratoconjunctivitis (VKC) is an ocular allergic disease characterized by a type 2 inflammation, tissue remodeling, and low quality of life for the affected patients. We investigated the involvement of endoplasmic reticulum (ER) stress and unfolded protein response in VKC.

Methods

Conjunctival imprints from VKC patients and normal subjects (CTs) were collected, and RNA was isolated, reverse transcribed, and analyzed with the Affymetrix microarray. Differentially expressed genes between VKC patients and CTs were evaluated. Genes related to ER stress, apoptosis, and autophagy were further considered. VKC and CT conjunctival biopsies were analyzed by immunohistochemistry (IHC) with specific antibodies against unfolded protein response (UPR), apoptosis, and inflam

SUBMITTER: Leonardi A 

PROVIDER: S-EPMC11008754 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature