Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis.

ABSTRACT: While cellular metabolism was proposed to be a driving factor of the activation and differentiation of B cells and the function of the resulting antibody-secreting cells (ASCs), the study of correlations between cellular metabolism and functionalities has been difficult due to the absence of technologies enabling the parallel measurement. Herein, we performed single-cell transcriptomics and introduced a direct concurrent functional and metabolic flux quantitation of individual murine B cells. Our transcriptomic data identified lactate metabolism as dynamic in ASCs, but antibody secretion did not correlate with lactate secretion rates (LSRs). Instead, our study of all splenic B cells during an immune response linked increased lactate metabolism with acidic intracellular pH and the upregulat

SUBMITTER: Bucheli OTM 

PROVIDER: S-EPMC11009249 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature