A simple and efficient in planta transformation method based on the active regeneration capacity of plants.

ABSTRACT: Plant genetic transformation strategies serve as essential tools for the genetic engineering and advanced molecular breeding of plants. However, the complicated operational protocols and low efficiency of current transformation strategies restrict the genetic modification of most plant species. This paper describes the development of the regenerative activity-dependent in planta injection delivery (RAPID) method based on the active regeneration capacity of plants. In this method, Agrobacterium tumefaciens is delivered to plant meristems via injection to induce transfected nascent tissues. Stable transgenic plants can be obtained by subsequent vegetative propagation of the positive nascent tissues. The method was successfully used for transformation of plants with strong regeneration capaci

SUBMITTER: Mei G 

PROVIDER: S-EPMC11009361 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature