Hydrophobic mismatch drives self-organization of designer proteins into synthetic membranes.

ABSTRACT: The organization of membrane proteins between and within membrane-bound compartments is critical to cellular function. Yet we lack approaches to regulate this organization in a range of membrane-based materials, such as engineered cells, exosomes, and liposomes. Uncovering and leveraging biophysical drivers of membrane protein organization to design membrane systems could greatly enhance the functionality of these materials. Towards this goal, we use de novo protein design, molecular dynamic simulations, and cell-free systems to explore how membrane-protein hydrophobic mismatch could be used to tune protein cotranslational integration and organization in synthetic lipid membranes. We find that membranes must deform to accommodate membrane-protein hydrophobic mismatch, which reduces the exp

SUBMITTER: Peruzzi JA 

PROVIDER: S-EPMC11009411 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature