Long-term imaging and spatio-temporal control of living cells using targeted light based on closed-loop feedback.

ABSTRACT: The ability to optically interact with cells on both an individual and collective level has applications from wound healing to cancer treatment. Building systems that can facilitate both localised light illumination and visualisation of cells can, however, be challenging and costly. This work takes the Dynamic Optical MicroEnvironment (DOME), an existing platform for the closed-loop optical control of microscale agents, and adapts the design to support live-cell imaging. Through modifications made to the imaging and projection systems within the DOME, a significantly higher resolution, alternative imaging channels and the ability to customise light wavelengths are achieved (Bio-DOME). This is accompanied by an interactive calibration procedure that is robust to changes in the hardware conf

SUBMITTER: Wijewardhane N 

PROVIDER: S-EPMC11009755 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature