DNA methylation of microRNA-365-1 induces apoptosis of hair follicle stem cells by targeting DAP3.

ABSTRACT:

Background

DNA methylation is a crucial epigenetic alteration involved in diverse biological processes and diseases. Nevertheless, the precise role of DNA methylation in chemotherapeutic drug-induced alopecia remains unclear. This study examined the role and novel processes of DNA methylation in regulating of chemotherapeutic drug-induced alopecia.

Methods

A mouse model of cyclophosphamide (CTX)-induced alopecia was established. Hematoxylin-eosin staining and immunohistochemical staining for the Ki67 proportion and a mitochondrial membrane potential assay (JC-1) were performed to assess the structural integrity and proliferative efficiency of the hair follicle stem cells (HFSCs). Immunofluorescence staining and real-time fluorescence quantitative PCR (RT-qPCR) were performed

SUBMITTER: Liu X 

PROVIDER: S-EPMC11010783 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature