Insights into the Adolescent Cystic Fibrosis Airway Microbiome Using Shotgun Metagenomics.

ABSTRACT: Cystic fibrosis (CF) is an inherited genetic disorder which manifests primarily in airway disease. Recent advances in molecular technologies have unearthed the diverse polymicrobial nature of the CF airway. Numerous studies have characterised the genus-level composition of this airway community using targeted 16S rDNA sequencing. Here, we employed whole-genome shotgun metagenomics to provide a more comprehensive understanding of the early CF airway microbiome. We collected 48 sputum samples from 11 adolescents and children with CF over a 12-month period and performed shotgun metagenomics on the Illumina NextSeq platform. We carried out functional and taxonomic analysis of the lung microbiome at the species and strain levels. Correlations between microbial diversity measures and independent

SUBMITTER: McDermott G 

PROVIDER: S-EPMC11011389 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature