The mRNA-Binding Protein KSRP Limits the Inflammatory Response of Macrophages.

ABSTRACT: KH-type splicing regulatory protein (KSRP) is a single-stranded nucleic acid-binding protein with multiple functions. It is known to bind AU-rich motifs within the 3'-untranslated region of mRNA species, which in many cases encode dynamically regulated proteins like cytokines. In the present study, we investigated the role of KSRP for the immunophenotype of macrophages using bone marrow-derived macrophages (BMDM) from wild-type (WT) and KSRP^-/- mice. RNA sequencing revealed that KSRP^-/- BMDM displayed significantly higher mRNA expression levels of genes involved in inflammatory and immune responses, particularly type I interferon responses, following LPS stimulation. In line, time kinetics studies revealed increased levels of interferon-γ (IFN-γ), interleukin (IL)-1β

SUBMITTER: Bolduan V 

PROVIDER: S-EPMC11011855 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature