An Ensemble Docking Approach for Analyzing and Designing Aptamer Heterodimers Targeting VEGF₁₆₅.

ABSTRACT: Vascular endothelial growth factor 165 (VEGF₁₆₅) is a prominent isoform of the VEGF-A protein that plays a crucial role in various angiogenesis-related diseases. It is homodimeric, and each of its monomers is composed of two domains connected by a flexible linker. DNA aptamers, which have emerged as potent therapeutic molecules for many proteins with high specificity and affinity, can also work for VEGF₁₆₅. A DNA aptamer heterodimer composed of monomers of V7t1 and del5-1 connected by a flexible linker (V7t1:del5-1) exhibits a greater binding affinity with VEGF₁₆₅ compared to either of the two monomers alone. Although the structure of the complex formed between the aptamer heterodimer and VEGF₁₆₅ is unknown due to the highly flexible linkers, gai

SUBMITTER: Go YJ 

PROVIDER: S-EPMC11012306 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature