Single-molecule characterization of Sen1 translocation properties provides insights into eukaryotic factor-dependent transcription termination.

ABSTRACT: Sen1 is an essential helicase for factor-dependent transcription termination in Saccharomyces cerevisiae, whose molecular-motor mechanism has not been well addressed. Here, we use single-molecule experimentation to better understand the molecular-motor determinants of its action on RNA polymerase II (Pol II) complex. We quantify Sen1 translocation activity on single-stranded DNA (ssDNA), finding elevated translocation rates, high levels of processivity and ATP affinities. Upon deleting the N- and C-terminal domains, or further deleting different parts of the prong subdomain, which is an essential element for transcription termination, Sen1 displays changes in its translocation properties, such as slightly reduced translocation processivities, enhanced translocation rates and statistically

SUBMITTER: Wang S 

PROVIDER: S-EPMC11013386 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature