Bioorthogonal labeling and profiling of N6-isopentenyladenosine (i6A) modified RNA.

ABSTRACT: Chemical modifications in RNAs play crucial roles in diversifying their structures and regulating numerous biochemical processes. Since the 1990s, several hydrophobic prenyl-modifications have been discovered in various RNAs. Prenyl groups serve as precursors for terpenes and many other biological molecules. The processes of prenylation in different macromolecules have been extensively studied. We introduce here a novel chemical biology toolkit that not only labels i6A, a prenyl-modified RNA residue, by leveraging the unique reactivity of the prenyl group, but also provides a general strategy to incorporate fluorescence functionalities into RNAs for molecular tracking purposes. Our findings revealed that iodine-mediated cyclization reactions of the prenyl group occur rapidly, transforming

SUBMITTER: Li Y 

PROVIDER: S-EPMC11014277 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature