Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology.

ABSTRACT: Regulated start-codon selection has the potential to reshape the proteome through the differential production of uORFs, canonical proteins, and alternative translational isoforms. However, conditions under which start-codon selection is altered remain poorly defined. Here, using transcriptome-wide translation initiation site profiling, we reveal a global increase in the stringency of start-codon selection during mammalian mitosis. Low-efficiency initiation sites are preferentially repressed in mitosis, resulting in pervasive changes in the translation of thousands of start sites and their corresponding protein products. This increased stringency of start-codon selection during mitosis results from increased interactions between the key regulator of start-codon selection, eIF1, and the 40S

SUBMITTER: Ly J 

PROVIDER: S-EPMC11014515 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature