Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases.

ABSTRACT: The gut microbiome possesses numerous biochemical enzymes that biosynthesize metabolites that impact human health. Bile acids comprise a diverse collection of metabolites that have important roles in metabolism and immunity. The gut microbiota-associated enzyme that is responsible for the gateway reaction in bile acid metabolism is bile salt hydrolase (BSH), which controls the host's overall bile acid pool. Despite the critical role of these enzymes, the ability to profile their activities and substrate preferences remains challenging due to the complexity of the gut microbiota, whose metaproteome includes an immense diversity of protein classes. Using a systems biochemistry approach employing activity-based probes, we have identified gut microbiota-associated BSHs that exhibit distinct su

SUBMITTER: Han L 

PROVIDER: S-EPMC11014516 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature